Journal: Materials & Design

Article Title: Multifunctional nanofiber-based dressings in coordination with adipose-derived stem cells for accelerated burn wound healing

doi: 10.1016/j.matdes.2025.113929

Figure Lengend Snippet: Fig. 4. (A) Detection of ADSCs proliferation by ADM coating at different times. Quantification of (B) EGF and (C) FGF2 in ADSCs paracrine products. *** P < 0.001 representing a significant difference as compared with ADM (3 min) and TLWDA.

Article Snippet: Human EGF enzyme-linked immunosorbent assay (ELISA) kit and human FGF2 ELISA kit were purchased from BOSTER (Wuhan, China).

Techniques:

Journal: BMJ Open

Article Title: Association between serum vitamin B 6 concentration and risk of osteoporosis in the middle-aged and older people in China: a cross-sectional study

doi: 10.1136/bmjopen-2018-028129

Figure Lengend Snippet: Comparison of serum Vit B 6 and serological metabolism parameters among control, osteopenia and osteoporosis groups

Article Snippet: The concentration of fibroblast growth factor 23 (FGF23) was measured through an ELISA performed using a human FGF23 ELISA kit (CSB-E10113h, Cusabio Biotech, Wuhan, China) and a micro plate reader (MK3, Thermo, Waltham, Massachusetts, USA).

Techniques: Comparison, Control

Journal: BMJ Open

Article Title: Association between serum vitamin B 6 concentration and risk of osteoporosis in the middle-aged and older people in China: a cross-sectional study

doi: 10.1136/bmjopen-2018-028129

Figure Lengend Snippet: Relationship between serum Vit B 6 concentrations and bone metabolism parameter concentrations in control, osteopenia and osteoporosis groups

Article Snippet: The concentration of fibroblast growth factor 23 (FGF23) was measured through an ELISA performed using a human FGF23 ELISA kit (CSB-E10113h, Cusabio Biotech, Wuhan, China) and a micro plate reader (MK3, Thermo, Waltham, Massachusetts, USA).

Techniques: Control

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: IHC staining of mp53 in SCCHN samples shows mainly nuclear and some cytoplasmic expression patterns. IHC staining of FGFR3 shows membrane and cytoplasmic expression. Four representative images are presented: mp53+/FGFR3+, mp53+/FGFR3-, mp53-/FGFR3+, mp53-/FGFR3- and negative control. (200 x magnification).

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques: Immunohistochemistry, Expressing, Membrane, Negative Control

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Univariate association of FGFR3 with clinical characteristics in cohort 1.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: FGFR3 association with clinical characteristics in cohort 2.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Correlation of FGFR3 with p53 and mp53 in cohort 1.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Pearson correlation coefficients (p-values) between FGFR3 and mp53, cytoplasmic and nuclear mp53 when p16 is negative.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Pearson correlation coefficients (p-values) between FGFR3 and mp53, cytoplasmic and nuclear mp53 when p16 is positive.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Cohort 1, univariate analysis of overall survival.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Cohort 1, univariate analysis of disease free survival.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Multivariate survival analysis on disease free survival.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: KM plots were generated using median weighted index (WI) of mp53 (3.5) and FGFR3 (35) as the cut off values (>median as positive and ≤ median as negative). High FGFR3 and mp53 levels correlated with worse OS (A) and DFS (B).

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques: Generated

Journal: International Journal of Molecular Sciences

Article Title: A Novel Recombinant Human FGF21 Analog with High Glycosylation Has a Prolonged Half-Life and Affects Glycemic and Body Weight Control

doi: 10.3390/ijms26062672

Figure Lengend Snippet: Construction, purification, and characterization of FGF21-164. ( A ) FGF21-164 sequence and FGF21 sequence. In the FGF21-164 sequence, red marked the mutated amino acid and blue represents the TR acid sequence from hCD164. In the FGF21 sequence, the red-labeled amino acid represents the amino acid sequence whose n-terminal is deleted in FGF21-164. ( B ) Subcloned cell culture supernatants expressing FGF21-164 and FGF21 were analyzed using Western blotting (Lane M: molecular weight marker. Lane 1~7: FGF21-164 clones 1, 2, 3, A4, B2, F3, and H4. Line 8: FGF21 clone2). ( C ) The purified FGF21-164 and FGF21 were assayed using SDS-PAGE and Western blotting (Lane M: molecular weight marker. Lane 1: purified FGF21-164. Line 2: purified FGF21). ( D ) Purity and hydrodynamic radius of FGF21-164 were analyzed using SEC-HPLC. ( E ) MALDI-TOF mass-spectrometry analysis of FGF21-164. ([M + H] + denotes the singly charged ionic forms, and [M + 2H] 2+ denotes doubly charged ionic forms). ( F ) Circular Dichroism (CD) spectra of FGF21-164 and FGF21.

Article Snippet: Here, we describe a novel human FGF21 analog, designated as FGF21-164, which was generated through fusion protein technology and co-encoded by a mutated FGF21 gene and the TR gene of hCD164.

Techniques: Purification, Sequencing, Labeling, Cell Culture, Expressing, Western Blot, Molecular Weight, Marker, Clone Assay, SDS Page, Mass Spectrometry, Circular Dichroism

Journal: International Journal of Molecular Sciences

Article Title: A Novel Recombinant Human FGF21 Analog with High Glycosylation Has a Prolonged Half-Life and Affects Glycemic and Body Weight Control

doi: 10.3390/ijms26062672

Figure Lengend Snippet: Glycan forms analysis of the protein FGF21-164. ( A ) O-linked glycan forms of FGF21-164. ( B ) N-linked glycan forms of FGF21-164.

Article Snippet: Here, we describe a novel human FGF21 analog, designated as FGF21-164, which was generated through fusion protein technology and co-encoded by a mutated FGF21 gene and the TR gene of hCD164.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: A Novel Recombinant Human FGF21 Analog with High Glycosylation Has a Prolonged Half-Life and Affects Glycemic and Body Weight Control

doi: 10.3390/ijms26062672

Figure Lengend Snippet: Effect of FGF21-164 in adipocytes induced by 3T3-L1. ( A ) The process of inducing 3T3-L1’s differentiation into adipocytes. ( B ) Reduction in glucose in medium stimulated by FGF21-164 in adipocytes induced by 3T3-L1. Data are means ± SEM (n = 6). * p < 0.05, *** p < 0.001, and **** p < 0.0001 compared to 0 μg/mL FGF21-164. ( C ) Phospho-Erk1/2-specific bands (Thr202/Tyr204; 44–42 kDa) were detected in 3T3-L1-derived adipocytes after FGF21-164 stimulation. ( D ) Evaluation of lipid droplet accumulation in adipocytes induced by 3T3-L1 with or without FGF21-164. The scale bar represents 50 μm.Data are the means ± SEM (n = 6). * p < 0.05, **** p < 0.0001.

Article Snippet: Here, we describe a novel human FGF21 analog, designated as FGF21-164, which was generated through fusion protein technology and co-encoded by a mutated FGF21 gene and the TR gene of hCD164.

Techniques: Derivative Assay

Journal: International Journal of Molecular Sciences

Article Title: A Novel Recombinant Human FGF21 Analog with High Glycosylation Has a Prolonged Half-Life and Affects Glycemic and Body Weight Control

doi: 10.3390/ijms26062672

Figure Lengend Snippet: Pharmacokinetics of FGF21-164. FGF21-164 plasma levels in C57BL/6 mice as determined via targeted LC-MS after i.v. (tail vein) bolus injection of FGF21-164 protein.

Article Snippet: Here, we describe a novel human FGF21 analog, designated as FGF21-164, which was generated through fusion protein technology and co-encoded by a mutated FGF21 gene and the TR gene of hCD164.

Techniques: Liquid Chromatography with Mass Spectroscopy, Injection

Journal: International Journal of Molecular Sciences

Article Title: A Novel Recombinant Human FGF21 Analog with High Glycosylation Has a Prolonged Half-Life and Affects Glycemic and Body Weight Control

doi: 10.3390/ijms26062672

Figure Lengend Snippet: The results of an oral glucose tolerance test applied to ob / ob mice after a single FGF21-164 treatment. ( A ) Blood glucose levels were measured at 0, 2, 3, 4, 5, 6, 7, and 8 h after the administration of FGF21-164. Data are the means ± SEM (n = 4–5). * p < 0.05 and ** p < 0.01 compared with the model. ( B ) The decrease in blood glucose levels post-administration relative to the baseline measurement at 0 h. Data are the means ± SEM (n = 4–5). * p < 0.05 compared with the model.

Article Snippet: Here, we describe a novel human FGF21 analog, designated as FGF21-164, which was generated through fusion protein technology and co-encoded by a mutated FGF21 gene and the TR gene of hCD164.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: A Novel Recombinant Human FGF21 Analog with High Glycosylation Has a Prolonged Half-Life and Affects Glycemic and Body Weight Control

doi: 10.3390/ijms26062672

Figure Lengend Snippet: Effect of a single administration of FGF21-164 on ob / ob mice. ( A , D , G ) Fasting glucose levels. Blood glucose levels were measured at 7, 21, and 28 days after a single dose of FGF21-164 was administered. Data are the means ± SEM (n = 4–5). ns: no significance. * p < 0.05, ** p < 0.01, and **** p < 0.0001. ( B , E , H ) The OGTT at 7, 21, and 28 days. After oral glucose administration, blood glucose levels were measured. Data are the means ± SEM (n = 4–5). * p < 0.05, *** p < 0.001, and **** p < 0.0001 at 6 mg·kg −1 vs. model. # p < 0.05, ## p < 0.01 and #### p < 0.0001 at 12 mg·kg −1 vs. model. ( C , F , I ) The glucose AUC during the OGTT process. Data are the means ± SEM (n = 4–5). ns: no significance. * p < 0.05, *** p < 0.001. ( J ) Body weight measured at 0, 7, 21, and 28 days. Data are the means ± SEM (n = 4–5). ns: no significance.

Article Snippet: Here, we describe a novel human FGF21 analog, designated as FGF21-164, which was generated through fusion protein technology and co-encoded by a mutated FGF21 gene and the TR gene of hCD164.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: A Novel Recombinant Human FGF21 Analog with High Glycosylation Has a Prolonged Half-Life and Affects Glycemic and Body Weight Control

doi: 10.3390/ijms26062672

Figure Lengend Snippet: Effect of repeated administration of FGF21-164. ( A ) Body weight changes. The DIO mice were treated with PBS or FGF21-164 once every 2 days. Data are the means ± SEM (n = 5–7). **** p < 0.0001 vs. Ctrl. #### p < 0.0001 for FGF21-164 vs. the model. ( B ) The liver sections were stained with Oil-red-O. The scale bar represents 100 μm. DIO mice showed many red lipid droplets in hepatic tissue (indicated by the black arrow), while lipid droplet levels were lower after the FGF21-164 treatment.

Article Snippet: Here, we describe a novel human FGF21 analog, designated as FGF21-164, which was generated through fusion protein technology and co-encoded by a mutated FGF21 gene and the TR gene of hCD164.

Techniques: Staining